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The influence of a sperm preparation in the infectivity of Brucella melitensis was studied. Semen from one fertile animal was incubated with and without semen activators for 15 min at 37 degrees C. The percentage of motility was evaluated before and after sperm activation. The number of spermatozoa infected with B. melitensis was determined by microbiological assays. Semen was recovered from animals after sexual intercourse, and the mean motility was assessed by means of sperm optical devices. The percentage of spermatozoa motility increased significantly (P less than 0.01) after activation, suggesting a greater integrity of the cell membrane. The capacity of spermatozoa to retain the B. melitensis